Changes
On March 11, 2024 at 8:44:11 AM CDT, Yanique Campbell:
-
Changed value of field
datasetCitation
toMundy, C.J., Leu, E., Campbell, K., Galindo, V., Levasseur, M., Poulin, M., Gosselin, M. Intracellular nutrient storage during an ice algal spring bloom - Arctic-ICE 2012. https://doi.org/10.34992/q15a-1e88
in Arctic-ICE 2012 Intracellular Nutrients
f | 1 | { | f | 1 | { |
2 | "Creator": "Creator", | 2 | "Creator": "Creator", | ||
3 | "Date": "2024-03-06", | 3 | "Date": "2024-03-06", | ||
4 | "IdentifierType": "DOI", | 4 | "IdentifierType": "DOI", | ||
5 | "PublicationYear": "2024", | 5 | "PublicationYear": "2024", | ||
6 | "Publisher": "CanWIN", | 6 | "Publisher": "CanWIN", | ||
7 | "RelatedIdentifierType": "URL", | 7 | "RelatedIdentifierType": "URL", | ||
8 | "RelationType": "IsSupplementTo", | 8 | "RelationType": "IsSupplementTo", | ||
9 | "ResourceType": "sea ice core dissolved and particulate data", | 9 | "ResourceType": "sea ice core dissolved and particulate data", | ||
10 | "Rights": "Creative Commons Attribution 4.0 International", | 10 | "Rights": "Creative Commons Attribution 4.0 International", | ||
11 | "Version": "1.0", | 11 | "Version": "1.0", | ||
12 | "accessTerms": "CanWIN datasets are licensed individually, however | 12 | "accessTerms": "CanWIN datasets are licensed individually, however | ||
13 | most are licensed under the Creative Commons Attribution 4.0 | 13 | most are licensed under the Creative Commons Attribution 4.0 | ||
14 | International (CC BY 4.0) Public License. Details for the licence | 14 | International (CC BY 4.0) Public License. Details for the licence | ||
15 | applied can be found using the Licence URL link provided with each | 15 | applied can be found using the Licence URL link provided with each | ||
16 | dataset. \r\nBy using data and information provided on this site you | 16 | dataset. \r\nBy using data and information provided on this site you | ||
17 | accept the terms and conditions of the License. Unless otherwise | 17 | accept the terms and conditions of the License. Unless otherwise | ||
18 | specified, the license grants the rights to the public to use and | 18 | specified, the license grants the rights to the public to use and | ||
19 | share the data and results derived therefrom as long as the proper | 19 | share the data and results derived therefrom as long as the proper | ||
20 | acknowledgment is given to the data licensor (citation), that any | 20 | acknowledgment is given to the data licensor (citation), that any | ||
21 | alteration to the data is clearly indicated, and that a link to the | 21 | alteration to the data is clearly indicated, and that a link to the | ||
22 | original data and the license is made available.", | 22 | original data and the license is made available.", | ||
23 | "activityCollectionType": "Field Measurement", | 23 | "activityCollectionType": "Field Measurement", | ||
24 | "analyticalInstrument": [ | 24 | "analyticalInstrument": [ | ||
25 | { | 25 | { | ||
26 | "Title": "", | 26 | "Title": "", | ||
27 | "analyticalInstrumentIdentifier": "", | 27 | "analyticalInstrumentIdentifier": "", | ||
28 | "identifierType": "", | 28 | "identifierType": "", | ||
29 | "name": "Cond 330i, WTW", | 29 | "name": "Cond 330i, WTW", | ||
30 | "titleType": "Alternative Title" | 30 | "titleType": "Alternative Title" | ||
31 | }, | 31 | }, | ||
32 | { | 32 | { | ||
33 | "Title": "", | 33 | "Title": "", | ||
34 | "analyticalInstrumentIdentifier": "", | 34 | "analyticalInstrumentIdentifier": "", | ||
35 | "identifierType": "", | 35 | "identifierType": "", | ||
36 | "name": "10-005R Turner Designs fluorometer", | 36 | "name": "10-005R Turner Designs fluorometer", | ||
37 | "titleType": "Alternative Title" | 37 | "titleType": "Alternative Title" | ||
38 | } | 38 | } | ||
39 | ], | 39 | ], | ||
40 | "analyticalMethod": [ | 40 | "analyticalMethod": [ | ||
41 | { | 41 | { | ||
42 | "analyticalMethodName": "Bulk Ice Salinity", | 42 | "analyticalMethodName": "Bulk Ice Salinity", | ||
43 | "comments": "", | 43 | "comments": "", | ||
44 | "laboratory": "", | 44 | "laboratory": "", | ||
45 | "methodLink": "", | 45 | "methodLink": "", | ||
46 | "methodSummary": "**Instrument**: Cond 330i, WTW\r\n\r\nMelt ice | 46 | "methodSummary": "**Instrument**: Cond 330i, WTW\r\n\r\nMelt ice | ||
47 | core without filtered seawater dilution and measure salinity at room | 47 | core without filtered seawater dilution and measure salinity at room | ||
48 | temperature.", | 48 | temperature.", | ||
49 | "variablesMeasured": "Salinity" | 49 | "variablesMeasured": "Salinity" | ||
50 | }, | 50 | }, | ||
51 | { | 51 | { | ||
52 | "analyticalMethodName": "Bottom ice chlorophyll (chl) a | 52 | "analyticalMethodName": "Bottom ice chlorophyll (chl) a | ||
53 | concentration\t", | 53 | concentration\t", | ||
54 | "comments": "", | 54 | "comments": "", | ||
55 | "laboratory": "", | 55 | "laboratory": "", | ||
56 | "methodLink": "", | 56 | "methodLink": "", | ||
57 | "methodSummary": "**Instrument**: 10-005R Turner Designs | 57 | "methodSummary": "**Instrument**: 10-005R Turner Designs | ||
58 | fluorometer\r\n\r\nMelted ice core samples were filtered onto Whatman | 58 | fluorometer\r\n\r\nMelted ice core samples were filtered onto Whatman | ||
59 | GF/F glass fiber filters (nominal pore size of 0.7 \u00b5m) for | 59 | GF/F glass fiber filters (nominal pore size of 0.7 \u00b5m) for | ||
60 | analysis of bottom-ice chl a. Filters were placed in 90% acetone for | 60 | analysis of bottom-ice chl a. Filters were placed in 90% acetone for | ||
61 | 18 to 24 hr, and the extracted chl a was measured before and after | 61 | 18 to 24 hr, and the extracted chl a was measured before and after | ||
62 | acidification with 5% HCl using a 10-005R Turner Designs fluorometer. | 62 | acidification with 5% HCl using a 10-005R Turner Designs fluorometer. | ||
63 | All measurements were made with ice core melt using 3:1 filtered | 63 | All measurements were made with ice core melt using 3:1 filtered | ||
64 | seawater dilution and corrected for the dilution.", | 64 | seawater dilution and corrected for the dilution.", | ||
65 | "variablesMeasured": "Chl a concentration" | 65 | "variablesMeasured": "Chl a concentration" | ||
66 | }, | 66 | }, | ||
67 | { | 67 | { | ||
68 | "analyticalMethodName": "Algal Taxonomy", | 68 | "analyticalMethodName": "Algal Taxonomy", | ||
69 | "comments": "", | 69 | "comments": "", | ||
70 | "laboratory": "", | 70 | "laboratory": "", | ||
71 | "methodLink": "", | 71 | "methodLink": "", | ||
72 | "methodSummary": "**Instrument**: Inverted | 72 | "methodSummary": "**Instrument**: Inverted | ||
73 | Microscope\t\r\n\r\nMelted ice core samples were preserved with acidic | 73 | Microscope\t\r\n\r\nMelted ice core samples were preserved with acidic | ||
74 | Lugol\u2019s solution (Parsons et al. 1984) and stored in the dark at | 74 | Lugol\u2019s solution (Parsons et al. 1984) and stored in the dark at | ||
75 | 4\u00b0C for later analysis of cell identification and enumeration. | 75 | 4\u00b0C for later analysis of cell identification and enumeration. | ||
76 | Cells > 4 \u00b5m were identified to the lowest possible taxonomic | 76 | Cells > 4 \u00b5m were identified to the lowest possible taxonomic | ||
77 | rank using inverted microscopy according to (Lund et al. 1958); | 77 | rank using inverted microscopy according to (Lund et al. 1958); | ||
78 | however, information is only presented on total autotrophic cell | 78 | however, information is only presented on total autotrophic cell | ||
79 | abundance and percent contribution of pennate diatoms. All | 79 | abundance and percent contribution of pennate diatoms. All | ||
80 | measurements were made with ice core melt using 3:1 filtered seawater | 80 | measurements were made with ice core melt using 3:1 filtered seawater | ||
81 | dilution and corrected for the | 81 | dilution and corrected for the | ||
82 | dilution.\r\n\r\n**References:**\r\n\r\n1. T. R. Parsons, Y. Maita, C. | 82 | dilution.\r\n\r\n**References:**\r\n\r\n1. T. R. Parsons, Y. Maita, C. | ||
83 | M. Lalli, A Manual of Chemical and Biological Methods for Seawater | 83 | M. Lalli, A Manual of Chemical and Biological Methods for Seawater | ||
84 | Analysis. (Pergamon Press, 1984) https:/doi.org/10.25607/OBP-1830 | 84 | Analysis. (Pergamon Press, 1984) https:/doi.org/10.25607/OBP-1830 | ||
85 | (March 4, 2024).\r\n\r\n2. J. W. G. Lund, C. Kipling, E. D. Le Cren, | 85 | (March 4, 2024).\r\n\r\n2. J. W. G. Lund, C. Kipling, E. D. Le Cren, | ||
86 | The inverted microscope method of estimating algal numbers and the | 86 | The inverted microscope method of estimating algal numbers and the | ||
87 | statistical basis of estimations by counting. Hydrobiologia 11, | 87 | statistical basis of estimations by counting. Hydrobiologia 11, | ||
88 | 143\u2013170 (1958).\t", | 88 | 143\u2013170 (1958).\t", | ||
89 | "variablesMeasured": "Percent contribution of main algal taxa" | 89 | "variablesMeasured": "Percent contribution of main algal taxa" | ||
90 | }, | 90 | }, | ||
91 | { | 91 | { | ||
92 | "analyticalMethodName": "Macronutrient concentrations", | 92 | "analyticalMethodName": "Macronutrient concentrations", | ||
93 | "comments": "", | 93 | "comments": "", | ||
94 | "laboratory": "", | 94 | "laboratory": "", | ||
95 | "methodLink": "", | 95 | "methodLink": "", | ||
96 | "methodSummary": "Sample was filtered through pre-combusted | 96 | "methodSummary": "Sample was filtered through pre-combusted | ||
97 | (450degC for 5 hr) Whatman GF/F filters using a sterilized syringe. | 97 | (450degC for 5 hr) Whatman GF/F filters using a sterilized syringe. | ||
98 | Filtrate was collected in acid-cleaned polyethylene tubes after three | 98 | Filtrate was collected in acid-cleaned polyethylene tubes after three | ||
99 | rinses with the filtrate, and stored at -20degC until analysis within | 99 | rinses with the filtrate, and stored at -20degC until analysis within | ||
100 | 6 months using a Bran-Luebbe 3 autoanalyzer (adapted from (Grasshoff | 100 | 6 months using a Bran-Luebbe 3 autoanalyzer (adapted from (Grasshoff | ||
101 | et al. 1999)). Samples were analyzed for nitrate+nitrite, phosphate | 101 | et al. 1999)). Samples were analyzed for nitrate+nitrite, phosphate | ||
102 | and silicic acid. Samples for Si(OH)4 determination were thawed for at | 102 | and silicic acid. Samples for Si(OH)4 determination were thawed for at | ||
103 | least 24 hr to minimize the issue of silicate polymerization when | 103 | least 24 hr to minimize the issue of silicate polymerization when | ||
104 | samples have been stored by freezing (Macdonald et al. | 104 | samples have been stored by freezing (Macdonald et al. | ||
105 | 1986).\r\n\r\n**Bulk ice nutrients** - samples were from ice cores | 105 | 1986).\r\n\r\n**Bulk ice nutrients** - samples were from ice cores | ||
106 | melted without filtered seawater addition\r\n\r\n**Water Column** - 2 | 106 | melted without filtered seawater addition\r\n\r\n**Water Column** - 2 | ||
107 | m water depth\r\n\r\n**Intracellular Nutrients** - The method used to | 107 | m water depth\r\n\r\n**Intracellular Nutrients** - The method used to | ||
108 | extract the intracellular nutrient pool was adapted from (Dortch | 108 | extract the intracellular nutrient pool was adapted from (Dortch | ||
109 | 1982). Within 3 hr of collection, a subsample from the scrape sample | 109 | 1982). Within 3 hr of collection, a subsample from the scrape sample | ||
110 | was filtered onto a pre-combusted (450\u00b0C for 5 h) Whatman GF/F | 110 | was filtered onto a pre-combusted (450\u00b0C for 5 h) Whatman GF/F | ||
111 | filter within an acid-cleaned filter head mounted on a large | 111 | filter within an acid-cleaned filter head mounted on a large | ||
112 | Erlenmeyer flask. Once enough material was concentrated on the filter | 112 | Erlenmeyer flask. Once enough material was concentrated on the filter | ||
113 | (visible confirmation), vacuum pressure was released and a 60-mL | 113 | (visible confirmation), vacuum pressure was released and a 60-mL | ||
114 | acid-cleaned polyethylene tube, rinsed with boiling reverse osmosis | 114 | acid-cleaned polyethylene tube, rinsed with boiling reverse osmosis | ||
115 | water, was suspended below the filtration head within the Erlenmeyer | 115 | water, was suspended below the filtration head within the Erlenmeyer | ||
116 | flask. Then, 40 mL of boiling reverse osmosis water was poured | 116 | flask. Then, 40 mL of boiling reverse osmosis water was poured | ||
117 | directly into the filter funnel. The water was left for 10 minutes and | 117 | directly into the filter funnel. The water was left for 10 minutes and | ||
118 | then vacuum pressure restored and the filtrate was collected in the | 118 | then vacuum pressure restored and the filtrate was collected in the | ||
119 | suspended tube. Following collection of the filtrate, the tube was | 119 | suspended tube. Following collection of the filtrate, the tube was | ||
120 | sealed and placed immediately into the -20degC freezer. Following the | 120 | sealed and placed immediately into the -20degC freezer. Following the | ||
121 | abovementioned protocol, a subsample of the boiling reverse osmosis | 121 | abovementioned protocol, a subsample of the boiling reverse osmosis | ||
122 | water was also collected as a blank for every sample day.\r\n\r\nQ. | 122 | water was also collected as a blank for every sample day.\r\n\r\nQ. | ||
123 | Dortch, Effect of growth conditions on accumulation of internal | 123 | Dortch, Effect of growth conditions on accumulation of internal | ||
124 | nitrate, ammonium, amino acids, and protein in three marine diatoms. | 124 | nitrate, ammonium, amino acids, and protein in three marine diatoms. | ||
125 | Journal of Experimental Marine Biology and Ecology 61, 243\u2013264 | 125 | Journal of Experimental Marine Biology and Ecology 61, 243\u2013264 | ||
126 | (1982).\r\n\r\nK. Grasssshoff, K. Kremling, M. Ehrhardt, | 126 | (1982).\r\n\r\nK. Grasssshoff, K. Kremling, M. Ehrhardt, | ||
127 | \u201cFrontmatter\u201d in Methods of Seawater Analysis, (John Wiley & | 127 | \u201cFrontmatter\u201d in Methods of Seawater Analysis, (John Wiley & | ||
128 | Sons, Ltd, 1999), pp. i\u2013xxxii.\r\n\r\nR. W. Macdonald, F. A. | 128 | Sons, Ltd, 1999), pp. i\u2013xxxii.\r\n\r\nR. W. Macdonald, F. A. | ||
129 | McLaughlin, C. S. Wong, The storage of reactive silicate samples by | 129 | McLaughlin, C. S. Wong, The storage of reactive silicate samples by | ||
130 | freezing. Limnology and Oceanography 31, 1139\u20131142 (1986).", | 130 | freezing. Limnology and Oceanography 31, 1139\u20131142 (1986).", | ||
131 | "variablesMeasured": "Macronutrient concentrations" | 131 | "variablesMeasured": "Macronutrient concentrations" | ||
132 | } | 132 | } | ||
133 | ], | 133 | ], | ||
134 | "author": null, | 134 | "author": null, | ||
135 | "author_email": null, | 135 | "author_email": null, | ||
136 | "awards": [ | 136 | "awards": [ | ||
137 | { | 137 | { | ||
138 | "awardTitle": "Discovery and Northern Research Supplements", | 138 | "awardTitle": "Discovery and Northern Research Supplements", | ||
139 | "awardURI": "", | 139 | "awardURI": "", | ||
140 | "funderIdentifierType": "", | 140 | "funderIdentifierType": "", | ||
141 | "funderName": "NSERC", | 141 | "funderName": "NSERC", | ||
142 | "funderSchemeURI": "" | 142 | "funderSchemeURI": "" | ||
143 | }, | 143 | }, | ||
144 | { | 144 | { | ||
145 | "awardTitle": "Network Project and Aircraft support", | 145 | "awardTitle": "Network Project and Aircraft support", | ||
146 | "awardURI": "", | 146 | "awardURI": "", | ||
147 | "funderIdentifierType": "", | 147 | "funderIdentifierType": "", | ||
148 | "funderName": "ArcticNet NCE", | 148 | "funderName": "ArcticNet NCE", | ||
149 | "funderSchemeURI": "" | 149 | "funderSchemeURI": "" | ||
150 | }, | 150 | }, | ||
151 | { | 151 | { | ||
152 | "awardTitle": "Start-up Grant (Mundy)", | 152 | "awardTitle": "Start-up Grant (Mundy)", | ||
153 | "awardURI": "", | 153 | "awardURI": "", | ||
154 | "funderIdentifierType": "", | 154 | "funderIdentifierType": "", | ||
155 | "funderName": "University of Manitoba", | 155 | "funderName": "University of Manitoba", | ||
156 | "funderSchemeURI": "" | 156 | "funderSchemeURI": "" | ||
157 | }, | 157 | }, | ||
158 | { | 158 | { | ||
159 | "awardTitle": "Logistical Support", | 159 | "awardTitle": "Logistical Support", | ||
160 | "awardURI": "", | 160 | "awardURI": "", | ||
161 | "funderIdentifierType": "", | 161 | "funderIdentifierType": "", | ||
162 | "funderName": "Polar Continental Shelf Project", | 162 | "funderName": "Polar Continental Shelf Project", | ||
163 | "funderSchemeURI": "" | 163 | "funderSchemeURI": "" | ||
164 | } | 164 | } | ||
165 | ], | 165 | ], | ||
166 | "campaignEndDate": "", | 166 | "campaignEndDate": "", | ||
167 | "campaignStartDate": "", | 167 | "campaignStartDate": "", | ||
168 | "contributorName": "Mundy, CJ", | 168 | "contributorName": "Mundy, CJ", | ||
169 | "contributorType": "DataCurator", | 169 | "contributorType": "DataCurator", | ||
170 | "contributors": [ | 170 | "contributors": [ | ||
171 | { | 171 | { | ||
172 | "affiliation": "Universit\u00e9 du Qu\u00e9bec \u00e0 Rimouski", | 172 | "affiliation": "Universit\u00e9 du Qu\u00e9bec \u00e0 Rimouski", | ||
173 | "contributorName": "Gosselin, Michel", | 173 | "contributorName": "Gosselin, Michel", | ||
174 | "contributorType": "ProjectMember", | 174 | "contributorType": "ProjectMember", | ||
175 | "email": "michel_gosselin@uqar.ca", | 175 | "email": "michel_gosselin@uqar.ca", | ||
176 | "nameIdentifier": "" | 176 | "nameIdentifier": "" | ||
177 | } | 177 | } | ||
178 | ], | 178 | ], | ||
179 | "creatorName": [ | 179 | "creatorName": [ | ||
180 | { | 180 | { | ||
181 | "author": "Mundy, CJ", | 181 | "author": "Mundy, CJ", | ||
182 | "creatorAffiliation": "Centre for Earth Observation Science - | 182 | "creatorAffiliation": "Centre for Earth Observation Science - | ||
183 | University of Manitoba", | 183 | University of Manitoba", | ||
184 | "creatorEmail": "cj.mundy@umanitoba.ca", | 184 | "creatorEmail": "cj.mundy@umanitoba.ca", | ||
185 | "creatorNameIdentifier": "0000-0001-5945-8305", | 185 | "creatorNameIdentifier": "0000-0001-5945-8305", | ||
186 | "nameIdentifierScheme": "ORCID", | 186 | "nameIdentifierScheme": "ORCID", | ||
187 | "nameType": "Personal", | 187 | "nameType": "Personal", | ||
188 | "schemeURI": "http://orcid.org/" | 188 | "schemeURI": "http://orcid.org/" | ||
189 | } | 189 | } | ||
190 | ], | 190 | ], | ||
191 | "creator_user_id": "59fdde0d-f226-4e5e-99ba-562b96c239a0", | 191 | "creator_user_id": "59fdde0d-f226-4e5e-99ba-562b96c239a0", | ||
192 | "dataCuratorAffiliation": "Centre for Earth Observation Science - | 192 | "dataCuratorAffiliation": "Centre for Earth Observation Science - | ||
193 | University of Manitoba", | 193 | University of Manitoba", | ||
194 | "dataCuratorEmail": "cj.mundy@umanitoba.ca", | 194 | "dataCuratorEmail": "cj.mundy@umanitoba.ca", | ||
195 | "datasetCitation": "Mundy, C.J., Leu, E., Campbell, K., Galindo, V., | 195 | "datasetCitation": "Mundy, C.J., Leu, E., Campbell, K., Galindo, V., | ||
196 | Levasseur, M., Poulin, M., Gosselin, M. Intracellular nutrient | 196 | Levasseur, M., Poulin, M., Gosselin, M. Intracellular nutrient | ||
n | 197 | storage during an ice algal spring bloom - Arctic-ICE | n | 197 | storage during an ice algal spring bloom - Arctic-ICE 2012. |
198 | 2012.10.34992/q15a-1e88", | 198 | https://doi.org/10.34992/q15a-1e88", | ||
199 | "datasetIdentifier": "10.34992/q15a-1e88", | 199 | "datasetIdentifier": "10.34992/q15a-1e88", | ||
200 | "datasetLevel": "1.5", | 200 | "datasetLevel": "1.5", | ||
201 | "datasetPublisher": "CanWIN", | 201 | "datasetPublisher": "CanWIN", | ||
202 | "dateType": "Updated", | 202 | "dateType": "Updated", | ||
203 | "descriptionType": "Abstract", | 203 | "descriptionType": "Abstract", | ||
204 | "eastBoundLongitude": "95.25", | 204 | "eastBoundLongitude": "95.25", | ||
205 | "embargoDate": "", | 205 | "embargoDate": "", | ||
206 | "endDate": "2012-06-08", | 206 | "endDate": "2012-06-08", | ||
207 | "endDateType": "Other", | 207 | "endDateType": "Other", | ||
208 | "frequency": "As needed", | 208 | "frequency": "As needed", | ||
n | 209 | "groups": [ | n | 209 | "groups": [], |
210 | { | ||||
211 | "description": "Features and characteristics of salt water | ||||
212 | bodies.\r\n\r\nIn CEOS, related research themes include | ||||
213 | biogeochemistry, modelling, marine mammals, oil spill response, | ||||
214 | physical oceanography, remote sensing and technology and trace metals | ||||
215 | and contaminants", | ||||
216 | "display_name": "Marine", | ||||
217 | "id": "98238b1c-5be8-41ad-8c6e-74cdc4f5f369", | ||||
218 | "image_display_url": | ||||
219 | ata/uploads/group/2021-10-31-211516.365746ofinspireoceanographic.svg", | ||||
220 | "name": "marine", | ||||
221 | "title": "Marine" | ||||
222 | } | ||||
223 | ], | ||||
224 | "id": "3c0b49c3-9f53-4930-8642-738495dcd4c8", | 210 | "id": "3c0b49c3-9f53-4930-8642-738495dcd4c8", | ||
225 | "isopen": false, | 211 | "isopen": false, | ||
226 | "kvSchemeURI": | 212 | "kvSchemeURI": | ||
227 | "https://www.polardata.ca/pdcinput/public/keywordlibrary", | 213 | "https://www.polardata.ca/pdcinput/public/keywordlibrary", | ||
228 | "licenceShemeURI": "https://spdx.org/licenses", | 214 | "licenceShemeURI": "https://spdx.org/licenses", | ||
229 | "licenceType": "Open", | 215 | "licenceType": "Open", | ||
230 | "license_id": null, | 216 | "license_id": null, | ||
231 | "license_title": null, | 217 | "license_title": null, | ||
232 | "maintainer": null, | 218 | "maintainer": null, | ||
233 | "maintainer_email": null, | 219 | "maintainer_email": null, | ||
234 | "metadata_created": "2024-03-07T19:07:32.668245", | 220 | "metadata_created": "2024-03-07T19:07:32.668245", | ||
t | 235 | "metadata_modified": "2024-03-11T13:42:15.253842", | t | 221 | "metadata_modified": "2024-03-11T13:44:11.201389", |
236 | "methodCitation": "", | 222 | "methodCitation": "", | ||
237 | "name": "arctic-ice-2012-intracellular-nutrients", | 223 | "name": "arctic-ice-2012-intracellular-nutrients", | ||
238 | "northBoundLatitude": "74.708", | 224 | "northBoundLatitude": "74.708", | ||
239 | "notes": "Nutrient availability influences maximum production, | 225 | "notes": "Nutrient availability influences maximum production, | ||
240 | speciation, cellular composition, and overall phenology of the Arctic | 226 | speciation, cellular composition, and overall phenology of the Arctic | ||
241 | spring ice algal bloom. However, how ice algae obtain nutrients from | 227 | spring ice algal bloom. However, how ice algae obtain nutrients from | ||
242 | their environment is not well-understood. Previously documented | 228 | their environment is not well-understood. Previously documented | ||
243 | positive relationships between sea ice nutrient concentrations and | 229 | positive relationships between sea ice nutrient concentrations and | ||
244 | algal biomass evidenced that ice algae maintain an intracellular | 230 | algal biomass evidenced that ice algae maintain an intracellular | ||
245 | nutrient pool. Here we provide direct evidence that **sea ice diatoms | 231 | nutrient pool. Here we provide direct evidence that **sea ice diatoms | ||
246 | store intracellular nitrate+nitrite and silicic acid well above that | 232 | store intracellular nitrate+nitrite and silicic acid well above that | ||
247 | available in their ambient environment**. Differential retention of | 233 | available in their ambient environment**. Differential retention of | ||
248 | intracellular pools released during standard melt processing | 234 | intracellular pools released during standard melt processing | ||
249 | techniques led to an increase in the apparent dissolved ratio of N:Si | 235 | techniques led to an increase in the apparent dissolved ratio of N:Si | ||
250 | measured in bulk ice melt samples that likely influenced | 236 | measured in bulk ice melt samples that likely influenced | ||
251 | interpretations of Si-limitation in some previous studies. The ability | 237 | interpretations of Si-limitation in some previous studies. The ability | ||
252 | of ice algae to store nutrient reserves also highlights a critical | 238 | of ice algae to store nutrient reserves also highlights a critical | ||
253 | biological process that stands to shift our understanding of nutrient | 239 | biological process that stands to shift our understanding of nutrient | ||
254 | dynamics in sea ice.\r\n", | 240 | dynamics in sea ice.\r\n", | ||
255 | "num_resources": 1, | 241 | "num_resources": 1, | ||
256 | "num_tags": 4, | 242 | "num_tags": 4, | ||
257 | "organization": { | 243 | "organization": { | ||
258 | "approval_status": "approved", | 244 | "approval_status": "approved", | ||
259 | "created": "2017-07-21T13:15:49.935872", | 245 | "created": "2017-07-21T13:15:49.935872", | ||
260 | "description": "The Centre for Earth Observation Science (CEOS) | 246 | "description": "The Centre for Earth Observation Science (CEOS) | ||
261 | was established in 1994 with a mandate to research, preserve and | 247 | was established in 1994 with a mandate to research, preserve and | ||
262 | communicate knowledge of Earth system processes using the technologies | 248 | communicate knowledge of Earth system processes using the technologies | ||
263 | of Earth Observation Science. Research is multidisciplinary and | 249 | of Earth Observation Science. Research is multidisciplinary and | ||
264 | collaborative seeking to understand the complex interrelationships | 250 | collaborative seeking to understand the complex interrelationships | ||
265 | between elements of Earth systems, and how these systems will likely | 251 | between elements of Earth systems, and how these systems will likely | ||
266 | respond to climate change. Although researchers have worked in many | 252 | respond to climate change. Although researchers have worked in many | ||
267 | regions, the Arctic marine system has always been a unifying focus of | 253 | regions, the Arctic marine system has always been a unifying focus of | ||
268 | activity.\r\n\r\nIn 2012, CEOS, along with the Greenland Climate | 254 | activity.\r\n\r\nIn 2012, CEOS, along with the Greenland Climate | ||
269 | Research Centre (GCRC, Nuuk, Greenland) and the Arctic Research Centre | 255 | Research Centre (GCRC, Nuuk, Greenland) and the Arctic Research Centre | ||
270 | (ARC, Aarhus, Denmark) established the Arctic Science Partnership, | 256 | (ARC, Aarhus, Denmark) established the Arctic Science Partnership, | ||
271 | thereby integrating academic and research initiatives.\r\n\r\nAreas of | 257 | thereby integrating academic and research initiatives.\r\n\r\nAreas of | ||
272 | existing research activity are divided among key themes:\r\n\r\nArctic | 258 | existing research activity are divided among key themes:\r\n\r\nArctic | ||
273 | Anthropology/Paleoclimatology: LiDAR scanning and digital site | 259 | Anthropology/Paleoclimatology: LiDAR scanning and digital site | ||
274 | preservation, archaeo-geophysics, permafrost degredation, lithic | 260 | preservation, archaeo-geophysics, permafrost degredation, lithic | ||
275 | morphometrics, zooarchaeology, proxy studies, paleodistribution of sea | 261 | morphometrics, zooarchaeology, proxy studies, paleodistribution of sea | ||
276 | ice, landscape learning, Paleo-Eskimo culture, Thule Inuit culture, | 262 | ice, landscape learning, Paleo-Eskimo culture, Thule Inuit culture, | ||
277 | ethnographic analogy, traditional knowledge, climate change and | 263 | ethnographic analogy, traditional knowledge, climate change and | ||
278 | northern heritage resource management.\r\n\r\nAtmospheric | 264 | northern heritage resource management.\r\n\r\nAtmospheric | ||
279 | Studies/Meteorology: Boundary layer, precipitation, clouds, storms and | 265 | Studies/Meteorology: Boundary layer, precipitation, clouds, storms and | ||
280 | extreme weather, circulation, eddy correlations, polar vortex, | 266 | extreme weather, circulation, eddy correlations, polar vortex, | ||
281 | climate, teleconnections, geophysical fluid dynamics, flux and energy | 267 | climate, teleconnections, geophysical fluid dynamics, flux and energy | ||
282 | budgets, ocean-sea ice-atmosphere interface, radiative transfer, ice | 268 | budgets, ocean-sea ice-atmosphere interface, radiative transfer, ice | ||
283 | albedo feedback, cloud radiative forcing, pCO2. | 269 | albedo feedback, cloud radiative forcing, pCO2. | ||
284 | \r\n\r\nBiogeochemistry: Organic carbon, greenhouse gases, bubbles, | 270 | \r\n\r\nBiogeochemistry: Organic carbon, greenhouse gases, bubbles, | ||
285 | Ikaite, carbonate chemistry, CO2 fluxes, mercury and other trace | 271 | Ikaite, carbonate chemistry, CO2 fluxes, mercury and other trace | ||
286 | metals, minerals, hydrocarbons, brine processes, otolith | 272 | metals, minerals, hydrocarbons, brine processes, otolith | ||
287 | microchemistry, sediments, biomarkers. \r\n\r\nContaminants: Mercury, | 273 | microchemistry, sediments, biomarkers. \r\n\r\nContaminants: Mercury, | ||
288 | trace metals, PAHs, source, transport, transformation, pathways, | 274 | trace metals, PAHs, source, transport, transformation, pathways, | ||
289 | bioaccumulations, marine ecosystems, marine chemistry. \r\nEarth | 275 | bioaccumulations, marine ecosystems, marine chemistry. \r\nEarth | ||
290 | Observation Science: Active and passive microwave, LiDAR, EM | 276 | Observation Science: Active and passive microwave, LiDAR, EM | ||
291 | induction, spatial-temporal analysis, forward and inverse scattering | 277 | induction, spatial-temporal analysis, forward and inverse scattering | ||
292 | models, complex permittivity, ocean colour, ocean surface roughness, | 278 | models, complex permittivity, ocean colour, ocean surface roughness, | ||
293 | NIR, TIR, satellite telemetry, GPS. Ice-Associated Biology: | 279 | NIR, TIR, satellite telemetry, GPS. Ice-Associated Biology: | ||
294 | Biophysical processes, primary production; ice algae, ice | 280 | Biophysical processes, primary production; ice algae, ice | ||
295 | microbiology, bio-optics, under-ice phytoplankton. \r\n\r\nInland | 281 | microbiology, bio-optics, under-ice phytoplankton. \r\n\r\nInland | ||
296 | Lakes and Waters: Hydrologic connectivity, watershed systems, sediment | 282 | Lakes and Waters: Hydrologic connectivity, watershed systems, sediment | ||
297 | transport, nutrient transport, contaminants, landscape processes, | 283 | transport, nutrient transport, contaminants, landscape processes, | ||
298 | remote sensing, freshwater-marine coupling. Marine Mammals: Seals, | 284 | remote sensing, freshwater-marine coupling. Marine Mammals: Seals, | ||
299 | whales, habitat, conservation, satellite telemetry, distribution, | 285 | whales, habitat, conservation, satellite telemetry, distribution, | ||
300 | population studies, prey behaviour, bioacoustics.\r\n\r\nModelling: | 286 | population studies, prey behaviour, bioacoustics.\r\n\r\nModelling: | ||
301 | Simulation of sea ice and oceanic regional processes, Nucleus for | 287 | Simulation of sea ice and oceanic regional processes, Nucleus for | ||
302 | European Modelling of the Ocean (NEMO), ice-ocean modelling and | 288 | European Modelling of the Ocean (NEMO), ice-ocean modelling and | ||
303 | interactions, hind cast simulations and projections for sea ice state | 289 | interactions, hind cast simulations and projections for sea ice state | ||
304 | and ocean variables based on CMIP5 scenarios and MIROC5 forcing, | 290 | and ocean variables based on CMIP5 scenarios and MIROC5 forcing, | ||
305 | validation.\r\n\r\nOceanography: Circulation, temperature, in-flow and | 291 | validation.\r\n\r\nOceanography: Circulation, temperature, in-flow and | ||
306 | out-flow shelves, water dynamics, microturbulence, Beaufort Gyre, eddy | 292 | out-flow shelves, water dynamics, microturbulence, Beaufort Gyre, eddy | ||
307 | correlations.\r\n\r\nSea Ice Geophysics:Thermodynamic and dynamic | 293 | correlations.\r\n\r\nSea Ice Geophysics:Thermodynamic and dynamic | ||
308 | processes, extreme ice features and hazards, snow, ridges, | 294 | processes, extreme ice features and hazards, snow, ridges, | ||
309 | polynyas.\r\n\r\nTraditional and Local Knowledge: Indigenous cultures, | 295 | polynyas.\r\n\r\nTraditional and Local Knowledge: Indigenous cultures, | ||
310 | Inuit, Inuvialuit, oral history, toponomy, mobility and settlement, | 296 | Inuit, Inuvialuit, oral history, toponomy, mobility and settlement, | ||
311 | hunting, food security, sea ice use, community-based research, | 297 | hunting, food security, sea ice use, community-based research, | ||
312 | community-based monitoring, two ways of knowing.", | 298 | community-based monitoring, two ways of knowing.", | ||
313 | "id": "9e21f6b6-d13f-4ba2-a379-fd962f507071", | 299 | "id": "9e21f6b6-d13f-4ba2-a379-fd962f507071", | ||
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315 | "is_organization": true, | 301 | "is_organization": true, | ||
316 | "name": "ceos", | 302 | "name": "ceos", | ||
317 | "state": "active", | 303 | "state": "active", | ||
318 | "title": "Centre for Earth Observation Science", | 304 | "title": "Centre for Earth Observation Science", | ||
319 | "type": "organization" | 305 | "type": "organization" | ||
320 | }, | 306 | }, | ||
321 | "owner_org": "9e21f6b6-d13f-4ba2-a379-fd962f507071", | 307 | "owner_org": "9e21f6b6-d13f-4ba2-a379-fd962f507071", | ||
322 | "private": false, | 308 | "private": false, | ||
323 | "projectImage": | 309 | "projectImage": | ||
324 | oba.ca/sites/default/files/users/user44/Bottom_Ice_Algae_Picture.png", | 310 | oba.ca/sites/default/files/users/user44/Bottom_Ice_Algae_Picture.png", | ||
325 | "publications": [ | 311 | "publications": [ | ||
326 | { | 312 | { | ||
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332 | "resourceType": "Online Resource" | 318 | "resourceType": "Online Resource" | ||
333 | } | 319 | } | ||
334 | ], | 320 | ], | ||
335 | "relatedIdentifier": "", | 321 | "relatedIdentifier": "", | ||
336 | "related_campaigns": [], | 322 | "related_campaigns": [], | ||
337 | "related_deployments": [], | 323 | "related_deployments": [], | ||
338 | "related_instruments": [], | 324 | "related_instruments": [], | ||
339 | "related_platforms": [], | 325 | "related_platforms": [], | ||
340 | "related_programs": [ | 326 | "related_programs": [ | ||
341 | "05fafa5f-cee8-4369-b6c3-8391d05dbbed" | 327 | "05fafa5f-cee8-4369-b6c3-8391d05dbbed" | ||
342 | ], | 328 | ], | ||
343 | "related_publications": [], | 329 | "related_publications": [], | ||
344 | "relationships_as_object": [], | 330 | "relationships_as_object": [], | ||
345 | "relationships_as_subject": [], | 331 | "relationships_as_subject": [], | ||
346 | "resourceTypeGeneral": "Dataset", | 332 | "resourceTypeGeneral": "Dataset", | ||
347 | "resources": [ | 333 | "resources": [ | ||
348 | { | 334 | { | ||
349 | "cache_last_updated": null, | 335 | "cache_last_updated": null, | ||
350 | "cache_url": null, | 336 | "cache_url": null, | ||
351 | "created": "2024-03-07T21:04:34.517284", | 337 | "created": "2024-03-07T21:04:34.517284", | ||
352 | "datastore_active": false, | 338 | "datastore_active": false, | ||
353 | "datastore_contains_all_records_of_source_file": false, | 339 | "datastore_contains_all_records_of_source_file": false, | ||
354 | "description": "Nutrient availability influences maximum | 340 | "description": "Nutrient availability influences maximum | ||
355 | production, speciation, cellular composition, and overall phenology of | 341 | production, speciation, cellular composition, and overall phenology of | ||
356 | the Arctic spring ice algal bloom. However, how ice algae obtain | 342 | the Arctic spring ice algal bloom. However, how ice algae obtain | ||
357 | nutrients from their environment is not well-understood. Previously | 343 | nutrients from their environment is not well-understood. Previously | ||
358 | documented positive relationships between sea ice nutrient | 344 | documented positive relationships between sea ice nutrient | ||
359 | concentrations and algal biomass evidenced that ice algae maintain an | 345 | concentrations and algal biomass evidenced that ice algae maintain an | ||
360 | intracellular nutrient pool. Here we provide direct evidence that sea | 346 | intracellular nutrient pool. Here we provide direct evidence that sea | ||
361 | ice diatoms store intracellular nitrate+nitrite and silicic acid well | 347 | ice diatoms store intracellular nitrate+nitrite and silicic acid well | ||
362 | above that available in their ambient environment. ", | 348 | above that available in their ambient environment. ", | ||
363 | "format": "XLSX", | 349 | "format": "XLSX", | ||
364 | "hash": "", | 350 | "hash": "", | ||
365 | "id": "e186270b-275f-4795-a3e0-5a3ec39f3e85", | 351 | "id": "e186270b-275f-4795-a3e0-5a3ec39f3e85", | ||
366 | "last_modified": "2024-03-07T21:04:34.431067", | 352 | "last_modified": "2024-03-07T21:04:34.431067", | ||
367 | "metadata_modified": "2024-03-07T21:13:46.407230", | 353 | "metadata_modified": "2024-03-07T21:13:46.407230", | ||
368 | "mimetype": null, | 354 | "mimetype": null, | ||
369 | "mimetype_inner": null, | 355 | "mimetype_inner": null, | ||
370 | "name": "IC Nutrients", | 356 | "name": "IC Nutrients", | ||
371 | "package_id": "3c0b49c3-9f53-4930-8642-738495dcd4c8", | 357 | "package_id": "3c0b49c3-9f53-4930-8642-738495dcd4c8", | ||
372 | "position": 0, | 358 | "position": 0, | ||
373 | "resCategory": "data", | 359 | "resCategory": "data", | ||
374 | "resource_type": null, | 360 | "resource_type": null, | ||
375 | "size": 22398, | 361 | "size": 22398, | ||
376 | "state": "active", | 362 | "state": "active", | ||
377 | "url": | 363 | "url": | ||
378 | 275f-4795-a3e0-5a3ec39f3e85/download/ic_nutrients_dataset_final.xlsx", | 364 | 275f-4795-a3e0-5a3ec39f3e85/download/ic_nutrients_dataset_final.xlsx", | ||
379 | "url_type": "upload" | 365 | "url_type": "upload" | ||
380 | } | 366 | } | ||
381 | ], | 367 | ], | ||
382 | "rightsIdentifier": "CC-BY-4.0", | 368 | "rightsIdentifier": "CC-BY-4.0", | ||
383 | "rightsIdentifierScheme": "SPDX", | 369 | "rightsIdentifierScheme": "SPDX", | ||
384 | "rightsURI": "https://spdx.org/licenses/CC-BY-4.0.html", | 370 | "rightsURI": "https://spdx.org/licenses/CC-BY-4.0.html", | ||
385 | "sample_collection": [ | 371 | "sample_collection": [ | ||
386 | { | 372 | { | ||
387 | "comment": "", | 373 | "comment": "", | ||
388 | "instrumentTitle": "Metre stick", | 374 | "instrumentTitle": "Metre stick", | ||
389 | "methodDescrioption": "", | 375 | "methodDescrioption": "", | ||
390 | "methodDescriptionType": "Methods", | 376 | "methodDescriptionType": "Methods", | ||
391 | "methodTitle": "Snow depth measurements", | 377 | "methodTitle": "Snow depth measurements", | ||
392 | "methodUrl": "", | 378 | "methodUrl": "", | ||
393 | "standardized_instrument_name": "metre stick" | 379 | "standardized_instrument_name": "metre stick" | ||
394 | }, | 380 | }, | ||
395 | { | 381 | { | ||
396 | "comment": "", | 382 | "comment": "", | ||
397 | "instrumentTitle": "Sea-Bird SBE 19plus V2 | 383 | "instrumentTitle": "Sea-Bird SBE 19plus V2 | ||
398 | conductivity-temperature-depth (CTD) probe", | 384 | conductivity-temperature-depth (CTD) probe", | ||
399 | "methodDescrioption": "2-m water depth salinities were extracted | 385 | "methodDescrioption": "2-m water depth salinities were extracted | ||
400 | from CTD casts.", | 386 | from CTD casts.", | ||
401 | "methodDescriptionType": "Methods", | 387 | "methodDescriptionType": "Methods", | ||
402 | "methodTitle": "Water column salinity", | 388 | "methodTitle": "Water column salinity", | ||
403 | "methodUrl": "", | 389 | "methodUrl": "", | ||
404 | "standardized_instrument_name": "Seabird CTD" | 390 | "standardized_instrument_name": "Seabird CTD" | ||
405 | }, | 391 | }, | ||
406 | { | 392 | { | ||
407 | "comment": "", | 393 | "comment": "", | ||
408 | "instrumentTitle": "Bran-Luebbe 3 autoanalyzer", | 394 | "instrumentTitle": "Bran-Luebbe 3 autoanalyzer", | ||
409 | "methodDescrioption": "Sample was filtered through pre-combusted | 395 | "methodDescrioption": "Sample was filtered through pre-combusted | ||
410 | (450degC for 5 hr) Whatman GF/F filters using a sterilized syringe. | 396 | (450degC for 5 hr) Whatman GF/F filters using a sterilized syringe. | ||
411 | Filtrate was collected in acid-cleaned polyethylene tubes after three | 397 | Filtrate was collected in acid-cleaned polyethylene tubes after three | ||
412 | rinses with the filtrate, and stored at -20degC until analysis within | 398 | rinses with the filtrate, and stored at -20degC until analysis within | ||
413 | 6 months using a Bran-Luebbe 3 autoanalyzer (adapted from (Grasshoff | 399 | 6 months using a Bran-Luebbe 3 autoanalyzer (adapted from (Grasshoff | ||
414 | et al. 1999)). Samples were analyzed for nitrate+nitrite, phosphate | 400 | et al. 1999)). Samples were analyzed for nitrate+nitrite, phosphate | ||
415 | and silicic acid. Samples for Si(OH)4 determination were thawed for at | 401 | and silicic acid. Samples for Si(OH)4 determination were thawed for at | ||
416 | least 24 hr to minimize the issue of silicate polymerization when | 402 | least 24 hr to minimize the issue of silicate polymerization when | ||
417 | samples have been stored by freezing (Macdonald et al. | 403 | samples have been stored by freezing (Macdonald et al. | ||
418 | 1986).\r\n\r\n**Bulk ice nutrients** - samples were from ice cores | 404 | 1986).\r\n\r\n**Bulk ice nutrients** - samples were from ice cores | ||
419 | melted without filtered seawater addition.\r\n\r\n**Water Column** - 2 | 405 | melted without filtered seawater addition.\r\n\r\n**Water Column** - 2 | ||
420 | m water depth\r\n\r\n**Intracellular Nutrients** - The method used to | 406 | m water depth\r\n\r\n**Intracellular Nutrients** - The method used to | ||
421 | extract the intracellular nutrient pool was adapted from (Dortch | 407 | extract the intracellular nutrient pool was adapted from (Dortch | ||
422 | 1982). Within 3 hr of collection, a subsample from the scrape sample | 408 | 1982). Within 3 hr of collection, a subsample from the scrape sample | ||
423 | was filtered onto a pre-combusted (450\u00b0C for 5 h) Whatman GF/F | 409 | was filtered onto a pre-combusted (450\u00b0C for 5 h) Whatman GF/F | ||
424 | filter within an acid-cleaned filter head mounted on a large | 410 | filter within an acid-cleaned filter head mounted on a large | ||
425 | Erlenmeyer flask. Once enough material was concentrated on the filter | 411 | Erlenmeyer flask. Once enough material was concentrated on the filter | ||
426 | (visible confirmation), vacuum pressure was released and a 60-mL | 412 | (visible confirmation), vacuum pressure was released and a 60-mL | ||
427 | acid-cleaned polyethylene tube, rinsed with boiling reverse osmosis | 413 | acid-cleaned polyethylene tube, rinsed with boiling reverse osmosis | ||
428 | water, was suspended below the filtration head within the Erlenmeyer | 414 | water, was suspended below the filtration head within the Erlenmeyer | ||
429 | flask. Then, 40 mL of boiling reverse osmosis water was poured | 415 | flask. Then, 40 mL of boiling reverse osmosis water was poured | ||
430 | directly into the filter funnel. The water was left for 10 minutes and | 416 | directly into the filter funnel. The water was left for 10 minutes and | ||
431 | then vacuum pressure restored and the filtrate was collected in the | 417 | then vacuum pressure restored and the filtrate was collected in the | ||
432 | suspended tube. Following collection of the filtrate, the tube was | 418 | suspended tube. Following collection of the filtrate, the tube was | ||
433 | sealed and placed immediately into the -20degC freezer. Following the | 419 | sealed and placed immediately into the -20degC freezer. Following the | ||
434 | above-mentioned protocol, a subsample of the boiling reverse osmosis | 420 | above-mentioned protocol, a subsample of the boiling reverse osmosis | ||
435 | water was also collected as a blank for every sample | 421 | water was also collected as a blank for every sample | ||
436 | day.\r\n\r\n\r\n** References**\r\n\r\n1. Q. Dortch, Effect of growth | 422 | day.\r\n\r\n\r\n** References**\r\n\r\n1. Q. Dortch, Effect of growth | ||
437 | conditions on accumulation of internal nitrate, ammonium, amino acids, | 423 | conditions on accumulation of internal nitrate, ammonium, amino acids, | ||
438 | and protein in three marine diatoms. Journal of Experimental Marine | 424 | and protein in three marine diatoms. Journal of Experimental Marine | ||
439 | Biology and Ecology 61, 243\u2013264 (1982).\r\n\r\n2. K. Grasssshoff, | 425 | Biology and Ecology 61, 243\u2013264 (1982).\r\n\r\n2. K. Grasssshoff, | ||
440 | K. Kremling, M. Ehrhardt, \u201cFrontmatter\u201d in Methods of | 426 | K. Kremling, M. Ehrhardt, \u201cFrontmatter\u201d in Methods of | ||
441 | Seawater Analysis, (John Wiley & Sons, Ltd, 1999), pp. | 427 | Seawater Analysis, (John Wiley & Sons, Ltd, 1999), pp. | ||
442 | i\u2013xxxii.\r\n\r\n3. R. W. Macdonald, F. A. McLaughlin, C. S. Wong, | 428 | i\u2013xxxii.\r\n\r\n3. R. W. Macdonald, F. A. McLaughlin, C. S. Wong, | ||
443 | The storage of reactive silicate samples by freezing. Limnology and | 429 | The storage of reactive silicate samples by freezing. Limnology and | ||
444 | Oceanography 31, 1139\u20131142 (1986).", | 430 | Oceanography 31, 1139\u20131142 (1986).", | ||
445 | "methodDescriptionType": "Methods", | 431 | "methodDescriptionType": "Methods", | ||
446 | "methodTitle": "Nutrient concentration", | 432 | "methodTitle": "Nutrient concentration", | ||
447 | "methodUrl": "", | 433 | "methodUrl": "", | ||
448 | "standardized_instrument_name": "" | 434 | "standardized_instrument_name": "" | ||
449 | }, | 435 | }, | ||
450 | { | 436 | { | ||
451 | "comment": "", | 437 | "comment": "", | ||
452 | "instrumentTitle": "Ice thickness tape", | 438 | "instrumentTitle": "Ice thickness tape", | ||
453 | "methodDescrioption": "Data were collected every 4 days between | 439 | "methodDescrioption": "Data were collected every 4 days between | ||
454 | 19 May and 8 June. Snow depths were measured at every core extraction | 440 | 19 May and 8 June. Snow depths were measured at every core extraction | ||
455 | location, with targeted sampling of three different sites to capture | 441 | location, with targeted sampling of three different sites to capture | ||
456 | the available range of snow depth conditions, including thin (<10 cm), | 442 | the available range of snow depth conditions, including thin (<10 cm), | ||
457 | medium (10-17 cm), and thick (>17 cm) snow covers. Bottom-ice samples | 443 | medium (10-17 cm), and thick (>17 cm) snow covers. Bottom-ice samples | ||
458 | were collected from each of these extraction locations using a Kovacs | 444 | were collected from each of these extraction locations using a Kovacs | ||
459 | Mark II coring system (9-cm inner diameter) and processed for analysis | 445 | Mark II coring system (9-cm inner diameter) and processed for analysis | ||
460 | of i) bottom-ice chlorophyll a concentration (chl a) and community | 446 | of i) bottom-ice chlorophyll a concentration (chl a) and community | ||
461 | composition, ii) intracellular nutrients and, iii) bottom-ice bulk | 447 | composition, ii) intracellular nutrients and, iii) bottom-ice bulk | ||
462 | nutrients.\r\n\r\nFor quantitative measurements of bottom-ice chl a | 448 | nutrients.\r\n\r\nFor quantitative measurements of bottom-ice chl a | ||
463 | and community composition, up to three ice cores were extracted from | 449 | and community composition, up to three ice cores were extracted from | ||
464 | each site and the bottom 3 cm were pooled into isothermal containers | 450 | each site and the bottom 3 cm were pooled into isothermal containers | ||
465 | before melt in 0.2-\uf06dm filtered seawater (FSW) to limit osmotic | 451 | before melt in 0.2-\uf06dm filtered seawater (FSW) to limit osmotic | ||
466 | shock to the algae during melt processing. The FSW-diluted core | 452 | shock to the algae during melt processing. The FSW-diluted core | ||
467 | solution was melted in the dark over a 15 to 20-hr period. For | 453 | solution was melted in the dark over a 15 to 20-hr period. For | ||
468 | intracellular nutrient measurements, a bottom-ice scrape sample was | 454 | intracellular nutrient measurements, a bottom-ice scrape sample was | ||
469 | collected from 1-3 cores per sampling site depending on visible algal | 455 | collected from 1-3 cores per sampling site depending on visible algal | ||
470 | coloration. The scrape procedure used a stainless-steel knife to | 456 | coloration. The scrape procedure used a stainless-steel knife to | ||
471 | scrape off the soft skeletal bottom-ice layer, which contained the | 457 | scrape off the soft skeletal bottom-ice layer, which contained the | ||
472 | strongest coloration of algal matter (<0.5 cm), directly into 500 mL | 458 | strongest coloration of algal matter (<0.5 cm), directly into 500 mL | ||
473 | of FSW at a temperature near freezing. This technique minimizes stress | 459 | of FSW at a temperature near freezing. This technique minimizes stress | ||
474 | on algal cells during ice melt processing by: i) maintaining sample | 460 | on algal cells during ice melt processing by: i) maintaining sample | ||
475 | salinities similar to growth conditions at the ice-ocean interface, | 461 | salinities similar to growth conditions at the ice-ocean interface, | ||
476 | and ii) reducing time of exposure to potentially stressful melt | 462 | and ii) reducing time of exposure to potentially stressful melt | ||
477 | conditions, as all scrape samples were processed within 3 hr of | 463 | conditions, as all scrape samples were processed within 3 hr of | ||
478 | collection. For bulk ice nutrient measurements, the bottom 3 cm of an | 464 | collection. For bulk ice nutrient measurements, the bottom 3 cm of an | ||
479 | ice core was collected and placed immediately into a sterile bag | 465 | ice core was collected and placed immediately into a sterile bag | ||
480 | (Nasco Whirl-Pak) and then melted over a 15 to 20-hr period in the | 466 | (Nasco Whirl-Pak) and then melted over a 15 to 20-hr period in the | ||
481 | dark.", | 467 | dark.", | ||
482 | "methodDescriptionType": "Methods", | 468 | "methodDescriptionType": "Methods", | ||
483 | "methodTitle": "Ice sample collection", | 469 | "methodTitle": "Ice sample collection", | ||
484 | "methodUrl": "", | 470 | "methodUrl": "", | ||
485 | "standardized_instrument_name": "" | 471 | "standardized_instrument_name": "" | ||
486 | }, | 472 | }, | ||
487 | { | 473 | { | ||
488 | "comment": "", | 474 | "comment": "", | ||
489 | "instrumentTitle": "Niskin sampler", | 475 | "instrumentTitle": "Niskin sampler", | ||
490 | "methodDescrioption": "A Niskin sampler was lowered through an | 476 | "methodDescrioption": "A Niskin sampler was lowered through an | ||
491 | ice hole to collect water at a 2-m depth.", | 477 | ice hole to collect water at a 2-m depth.", | ||
492 | "methodDescriptionType": "Methods", | 478 | "methodDescriptionType": "Methods", | ||
493 | "methodTitle": "Water sampling", | 479 | "methodTitle": "Water sampling", | ||
494 | "methodUrl": "", | 480 | "methodUrl": "", | ||
495 | "standardized_instrument_name": "Niskin Bottle" | 481 | "standardized_instrument_name": "Niskin Bottle" | ||
496 | } | 482 | } | ||
497 | ], | 483 | ], | ||
498 | "southBoundLatitude": "74.708", | 484 | "southBoundLatitude": "74.708", | ||
499 | "spatial": | 485 | "spatial": | ||
500 | 226],[-95.2923820456,74.801703226],[-95.2923820456,74.6279297473]]]}", | 486 | 226],[-95.2923820456,74.801703226],[-95.2923820456,74.6279297473]]]}", | ||
501 | "spatial_regions": "resolute", | 487 | "spatial_regions": "resolute", | ||
502 | "startDate": "2012-05-19", | 488 | "startDate": "2012-05-19", | ||
503 | "startDateType": "Collected", | 489 | "startDateType": "Collected", | ||
504 | "state": "active", | 490 | "state": "active", | ||
505 | "status": "Complete", | 491 | "status": "Complete", | ||
506 | "subjectScheme": "Polar Data Catalogue", | 492 | "subjectScheme": "Polar Data Catalogue", | ||
507 | "supplementalResources": [ | 493 | "supplementalResources": [ | ||
508 | { | 494 | { | ||
509 | "RelatedIdentifier": "", | 495 | "RelatedIdentifier": "", | ||
510 | "ResourceTypeGeneral": "", | 496 | "ResourceTypeGeneral": "", | ||
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517 | ], | 503 | ], | ||
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519 | { | 505 | { | ||
520 | "display_name": "Arctic", | 506 | "display_name": "Arctic", | ||
521 | "id": "ba65ee6f-85a0-49ba-a33f-4908378903c7", | 507 | "id": "ba65ee6f-85a0-49ba-a33f-4908378903c7", | ||
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525 | }, | 511 | }, | ||
526 | { | 512 | { | ||
527 | "display_name": "Ice algae", | 513 | "display_name": "Ice algae", | ||
528 | "id": "17ef24c8-9d33-4b66-86a3-593c36ceb4bb", | 514 | "id": "17ef24c8-9d33-4b66-86a3-593c36ceb4bb", | ||
529 | "name": "Ice algae", | 515 | "name": "Ice algae", | ||
530 | "state": "active", | 516 | "state": "active", | ||
531 | "vocabulary_id": null | 517 | "vocabulary_id": null | ||
532 | }, | 518 | }, | ||
533 | { | 519 | { | ||
534 | "display_name": "Nutrients", | 520 | "display_name": "Nutrients", | ||
535 | "id": "44265c2a-a639-4779-9481-478b0d6262ac", | 521 | "id": "44265c2a-a639-4779-9481-478b0d6262ac", | ||
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537 | "state": "active", | 523 | "state": "active", | ||
538 | "vocabulary_id": null | 524 | "vocabulary_id": null | ||
539 | }, | 525 | }, | ||
540 | { | 526 | { | ||
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542 | "id": "ac8365f3-118a-4786-886e-f96ad81ceb7c", | 528 | "id": "ac8365f3-118a-4786-886e-f96ad81ceb7c", | ||
543 | "name": "Taxonomy", | 529 | "name": "Taxonomy", | ||
544 | "state": "active", | 530 | "state": "active", | ||
545 | "vocabulary_id": null | 531 | "vocabulary_id": null | ||
546 | } | 532 | } | ||
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550 | ], | 536 | ], | ||
551 | "title": "Arctic-ICE 2012 Intracellular Nutrients", | 537 | "title": "Arctic-ICE 2012 Intracellular Nutrients", | ||
552 | "titleType": "Alternative Title", | 538 | "titleType": "Alternative Title", | ||
553 | "type": "dataset", | 539 | "type": "dataset", | ||
554 | "url": null, | 540 | "url": null, | ||
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559 | } | 545 | } |