The 2016 mooring field program took place in southern Hudson Bay from September 26 (Churchill) to October 4 (Kujjaurapik) (Figure 1). Opportunistic sampling continued from October 5 to October 12 in northern Hudson Bay (Figure 1), after which the ship returned to Iqaluit for crew change and all scientists disembarked. During the main eight-day cruise, members of all five multi-disciplinary teams collected CTD profiles, water and sediment samples,and deployed oceanographic moorings along the full length of the southern coast of Hudson Bay. The focus of this field program was on the Nelson Estuary region and James Bay mouth, which are the major sources of riverine fresh water to the Hudson Bay system.