Survey 1 took place from May 18 - June 16, 2016. Lakes Manitoba, Winnipegosis and Waterhen were sampled and underwater moorings deployed on all 3 lakes. Multiple rivers around the sample area were also sampled for surface water. Samples were taken at all sites for nutrients, biogeochemistry, physical variables (light), zooplankton and algae for taxonomy. At this time the Lowe boat did not have a depth finder so depth would have been taken from the Idronaut (CTD) records. Water samples were filtered either at Lake Waterhen cabin or at CEOS and sent to the Freshwater Institute for analysis.